RECOMBINANT PROTEINS / EPO

Erythropoietin (EPO) is a glycoprotein hormone best known for its role in stimulating bone marrow to produce red blood cells. Consequently, recombinant human EPO is an important biopharmaceutical drug for treatment of anemia. Four forms, namely epoetin alfa, epoetin beta, epoetin delta and darbepoetin alfa, are distinguished mainly by their glycosylation.

Recombinant EPO is produced using mammalian cell lines, such as CHO, which are engineered to express the protein. After cell culture and harvest, downstream purification is required to achieve the necessary product purity. A popular approach uses an ion exchange column followed by a reversed-phase C4 column step.

In addition to EPO, other recombinant proteins that are purified with silica gel include colony stimulating factors such as filgrastim and sargramostim.

silica gel for purification of recombinant proteins
SP-C4-P
EPO Purification Phase

EPO Purification Phase

Our P Series C4 silica gel is bonded with butyl groups to provide the moderate hydrophobicity that is best suited for the purification of erythropoietin and similar recombinant proteins. It is available in 300Å pore size to accommodate these large molecules.

Go to P Series

Bulk Silica Gel for Recombinant Protein Purification

The following DAISOGEL products are suitable for the purification of recombinant proteins such as EPO. Click on a pore size to learn more.

Recommended Pore Size (Å)
ODS Phase C8 Phase C4 Phase APS Phase
P Series 300

Did you know?

Even though the first epoetin alfa was approved back in 1989, the market is still increasing due to a rise in the number of patients suffering from anemic conditions induced from cancer, HIV, and other afflictions.