Screening Guide
Once the COMPASS has pointed you in the best direction for your molecule, you are ready to embark on your screening studies. It is crucial that the stationary phase that best achieves project goals be selected. Otherwise, the whole project will be off to a bad start. For some projects, the key goal may be product yield. For others, it may be upfront silica gel cost. For others, bulk silica delivery time and loading may be key. Here are some other considerations for screening columns:
- Base your screening selection on science. DO NOT simply use whatever stationary phase you have on hand!
- DO be impartial while screening columns. Use the same particle size, conditions, samples, and equipment.
Know Your Project Goals
Get your screening studies off on the right foot
Product
Yield
Upfront
Silica Gel Cost
Bulk Silica
Delivery Time
DAISOGEL Hint Charts
Screening so many columns and optimizing the process is very time consuming. Like many suppliers, DAISOGEL has a wide range of silica gels to choose from, each with their own nuances. To that end, DAISOGEL provides the following hint charts for our bonded phases to assist your pre-selection for screening based on your COMPASS results:
ODS Hint Chart
| DAISOGEL Bulk Silica Part No. | Silica Backbone | Bonding | Specialty | Comparable Products |
|---|---|---|---|---|
| SP-100-x-ODS-P | High surface area | Aqueous | Ideal for small molecules and synthetic peptides | Luna® C18 |
| SP-100-x-ODS-HP | High surface area | Very high C% | For difficult separations needing long retention | |
| SP-100-x-ODS-ZP | High surface area | Low hydrophobicity | Low C load on high surface area | Spheriorex |
| SP-120-x-ODS-BP | Standard | Aqueous | The perfect aqueous phase (limited pH range) | ODS-B |
| SP-120-x-ODS-RPS | Standard | Standard | Workhorse; the place to start process development | ODS-A |
| SP-120-x-ODS-BIO | Standard | Very high C% | High bonding density provides wide pH range | |
| SP-200-x-ODS-BP | Wider pore | Aqueous | The perfect aqueous phase (limited pH range) | |
| SP-200-x-ODS-RPS | Wider pore | Standard | Workhorse; the place to start process development | |
| SP-200-x-ODS-BIO | Wider pore | Very high C% | High bonding density provides wide pH range! | |
| SP-300-x-ODS-BP | Much wider pore | Aqueous | The perfect aqueous phase (limited pH range) | |
| SP-300-x-ODS-RPS | Much wider pore | Standard | Workhorse; the place to start process development | |
| SP-300-x-ODS-BIO | Much wider pore | Very high C% | High bonding density provides wide pH range! | |
| SP-100-x-ODS-PK | Calcinated | Very high C% | Mechanical strength with chemical resistance; TOUGH! | KROMASIL® Classic C18 |
C8 Hint Chart
| DAISOGEL Bulk Silica Part No. | Silica Backbone | Bonding | Specialty | Comparable Products |
|---|---|---|---|---|
| SP-100-x-C8-HP | High surface area | Very high C% | Works like magic for Orlistat | Luna® C18 |
| SP-120-x-C8-P | Standard | Standard | Reliable C8 phase | |
| SP-120-x-C8-BIO | Standard | Very high C% | Reliable C8 phase for wider pH range | |
| SP-200-x-C8-P | Wider pore | Standard | Reliable C8 phase for larger peptides | |
| SP-200-x-C8-BIO | Wider pore | Very high C% | The insulin phase | YMC basic |
| SP-100-x-C8-PK | Calcinated | Very high C% | The most robust insulin phase | KROMASIL® Classic C8 |
C4-Phenyl Hint Chart
| DAISOGEL Bulk Silica Part No. | Silica Backbone | Bonding | Specialty | Comparable Products |
|---|---|---|---|---|
| SP-100-x-C4Ph-HP | High surface area | Unique phenyl ligand | Alternative separation patterns via secondary interactions | Luna® PheHex |
C4 (non-endcapped) Hint Chart
| DAISOGEL Bulk Silica Part No. | Silica Backbone | Bonding | Specialty | Comparable Products |
|---|---|---|---|---|
| SP-100-x-C4-NP | High surface area | Non-endcapped | Unique balance between silanol interaction and C4 |
Luna® is a registered trademark of Phenomenex, Inc.
Kromasil® is a registered trademark of Nouryon in a number of territories in the world.
Need More Guidance for Your
Screening Studies?
Screening the most suitable phase for every new peptide purification process is important. We hope we can be of assistance with your complex and important task.
Reach out to DAISOGEL and we will gladly consult with you and walk you through the screening process with no obligations. We can help provide tips make shortcuts for you from our experience.