Screening Guide

Once the COMPASS has pointed you in the best direction for your molecule, you are ready to embark on your screening studies. It is crucial that the stationary phase that best achieves project goals be selected. Otherwise, the whole project will be off to a bad start. For some projects, the key goal may be product yield. For others, it may be upfront silica gel cost. For others, bulk silica delivery time and loading may be key. Here are some other considerations for screening columns:

  1. Base your screening selection on science. DO NOT simply use whatever stationary phase you have on hand!
  2. DO be impartial while screening columns. Use the same particle size, conditions, samples, and equipment.

Know Your Project Goals

Get your screening studies off on the right foot

Product
Yield

Upfront
Silica Gel Cost

Bulk Silica
Delivery Time

DAISOGEL Hint Charts

Screening so many columns and optimizing the process is very time consuming. Like many suppliers, DAISOGEL has a wide range of silica gels to choose from, each with their own nuances. To that end, DAISOGEL provides the following hint charts for our bonded phases to assist your pre-selection for screening based on your COMPASS results:

ODS Hint Chart

DAISOGEL
Bulk Silica Part No.
Silica BackboneBondingSpecialtyComparable Products
SP-100-x-ODS-PHigh surface areaAqueousIdeal for small molecules and synthetic peptidesLuna® C18
SP-100-x-ODS-HPHigh surface areaVery high C%For difficult separations needing long retention
SP-100-x-ODS-ZPHigh surface areaLow hydrophobicityLow C load on high surface areaSpheriorex
SP-120-x-ODS-BPStandardAqueousThe perfect aqueous phase (limited pH range)ODS-B
SP-120-x-ODS-RPSStandardStandardWorkhorse; the place to start process developmentODS-A
SP-120-x-ODS-BIOStandardVery high C%High bonding density provides wide pH range
SP-200-x-ODS-BPWider poreAqueousThe perfect aqueous phase  (limited pH range)
SP-200-x-ODS-RPSWider poreStandardWorkhorse; the place to start process development
SP-200-x-ODS-BIOWider poreVery high C%High bonding density provides wide pH range!
SP-300-x-ODS-BPMuch wider poreAqueousThe perfect aqueous phase (limited pH range)
SP-300-x-ODS-RPSMuch wider poreStandardWorkhorse; the place to start process development
SP-300-x-ODS-BIOMuch wider poreVery high C%High bonding density provides wide pH range!
SP-100-x-ODS-PKCalcinatedVery high C%Mechanical strength with chemical resistance; TOUGH!KROMASIL® Classic C18

C8 Hint Chart

DAISOGEL
Bulk Silica Part No.
Silica BackboneBondingSpecialtyComparable Products
SP-100-x-C8-HPHigh surface areaVery high C%Works like magic for OrlistatLuna® C18
SP-120-x-C8-PStandardStandardReliable C8 phase
SP-120-x-C8-BIOStandardVery high C%Reliable C8 phase for wider pH range
SP-200-x-C8-PWider poreStandardReliable C8 phase for larger peptides
SP-200-x-C8-BIOWider poreVery high C%The insulin phaseYMC basic
SP-100-x-C8-PKCalcinatedVery high C%The most robust insulin phaseKROMASIL® Classic C8

C4-Phenyl Hint Chart

DAISOGEL
Bulk Silica Part No.
Silica BackboneBondingSpecialtyComparable Products
SP-100-x-C4Ph-HPHigh surface areaUnique phenyl ligandAlternative separation patterns via secondary interactionsLuna® PheHex

C4 (non-endcapped) Hint Chart

DAISOGEL
Bulk Silica Part No.
Silica BackboneBondingSpecialtyComparable Products
SP-100-x-C4-NPHigh surface areaNon-endcappedUnique balance between silanol interaction and C4

Luna® is a registered trademark of Phenomenex, Inc.
Kromasil® is a registered trademark of Nouryon in a number of territories in the world.

Need More Guidance for Your
Screening Studies?

Screening the most suitable phase for every new peptide purification process is important. We hope we can be of assistance with your complex and important task.

Reach out to DAISOGEL and we will gladly consult with you and walk you through the screening process with no obligations. We can help provide tips make shortcuts for you from our experience.