Technical Notes

Your knowledgebase for DAISOGEL silica gel products

  • Approximate Packing Density for DAISOGEL Bulk Silica Gel.

    While we offer prepacked columns for analytical and prep scale, DAISOGEL bulk silica gel is most well-known for its availability in kilogram quantities for larger scale purifications. In such cases, we recommend packing your bulk silica gel in a Dynamic Axial Compression (DAC) column. When you are employing a DAC column (or self-packing device) and packing it yourself with DAISOGEL bulk silica, the goal is to have an evenly packed silica bed with the desired bed length. Due to the mechanical axial compression of the DAC columns, you must first measure out the appropriate amount of dry silica gel prior

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  • Which DAISOGEL grade is best for recombinant peptide purification?

    Recombinant peptides are therapeutics produced from the recombinant DNA method, a technique which allows for much longer and more complex peptides to be produced. Production starts with fermentation using a microbial host, such as the bacterial microorganism E. coli, or a yeast-based microorganism such as S. cerevisiae or, more recently, the methylotrophic Pichia pastoris. These microorganisms are engineered to express the recombinant peptide of interest during the fermentation process. Unlike the synthetic method of peptide production, the recombinant method produces a significant amount of host cell debris, glycosylated proteins, and other impurities that must be removed after harvest. In addition,

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  • Which DAISOGEL grade is best for synthetic peptide purification?

    While there isn’t one general answer, let’s start with some background on the different types of peptides and their makeup. In general, there are two upstream modes for large-scale production of peptide-based APIs: Synthetic method: solid-phase peptide synthesis (SPPS) using amino acid starting materials and either Boc or the milder Fmoc chemistry; Recombinant DNA method: recombinant production from microorganism culture such as E. coli Peptides produced using the synthetic method are generally very clean and do not require as much purification compared to the recombinant peptides. The recombinant DNA method enables the production of much longer and more complex peptides;

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